Expression of neurotransmitter receptors in oral keratinocytes and their response to agonists
최은지, 장성호, 최세영, 최영님,
소속 상세정보
최은지 ( Choi Eun-Ji ) - Seoul National University School of Dentistry Department of Oral Microbiology and Immunology
장성호 ( Chang Sung-Ho ) - Seoul National University School of Dentistry Department of Oral Microbiology and Immunology
최세영 ( Choi Se-Young ) - Seoul National University School of Dentistry Department of Neuroscience
최영님 ( Choi Young-Nim ) - Seoul National University School of Dentistry Department of Oral Microbiology and Immunology
Abstract
This study aimed to investigate whether neurotransmitter receptors in the nervous system were also expressed in oral keratinocytes. Expressions of various neurotransmitter receptor genes in immortalized mouse oral keratinocyte (IMOK) cells were examined by reverse transcriptase polymerase chain reaction. IMOK cells expressed calcitonin gene-related peptide (CGRP) receptor subunit genes Ramp1 and Ramp3 and glutamate receptor subunit genes Grina , Gria3 , Grin1 , Grin2a , and Grin2d . Moreover, IMOK cells expressed Adrb2 and Chrna5 that encode beta 2 adrenergic receptor and cholinergic receptor nicotinic alpha 5 for sympathetic and parasympathetic neurotransmitters, respectively. The expression of Bdkrb1 and Ptger4 , which encode receptors for bradykinin and prostaglandin E2 involved in inflammatory responses, was also observed at low levels. Expressions of Ramp1 and Grina in the mouse gingival epithelium were also confirmed by immunohistochemistry. When the function of neurotransmitter receptors expressed on IMOK cells was tested by intracellular calcium response, CGRP, glutamate, and cholinergic receptors did not respond to their agonists, but the bradykinin receptor responded to bradykinin. Collectively, oral keratinocytes express several neurotransmitter receptors, suggesting the potential regulation of oral epithelial homeostasis by the nervous system.
키워드
Nervous system; Neurotransmitter receptor; Keratinocyte; Mouth mucosa
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